Latest updates on WP2




Activities concerning the WP2 (metabolic engineering for biomass conversion to butanol) have been carried out by WEIZMANN. A library of ligno-cellulolytic enzymes has been developed and a panel of mixtures tested on the four different types of untreated agrowastes. High levels of enzymatic degradation was observed on brewer’s spent grain by a mixture of 3 cellulases, 1 xylanase and 1 bifunctional xylanase-laccase. These five enzymes were attached to scaffoldins to form a designer cellulosomal complex, which was found to be even more efficient than the free enzyme mixture.